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J Biosci ; 2003 Sep; 28(5): 589-95
Artigo em Inglês | IMSEAR | ID: sea-111079

RESUMO

A survey was conducted to study the biological and genetic diversity of Cardamom mosaic virus (CdMV) that causes the most widespread disease in the cardamom growing area in the Western Ghats of south India. Six distinct subgroups were derived based on their symptomatology and host range from the sixty isolates collected. The serological variability between the virus isolates was analysed by ELISA and Western blotting. The 3 terminal region consisting of the coat protein (CP) coding sequence and 3 untranslated region (3 UTR) was cloned and sequenced from seven isolates. Sequence comparisons revealed considerable genetic diversity among the isolates in their CP and 3 UTR, making CdMV one of the highly variable members of Potyviridae. The possible occurrence of recombination between the isolates and the movement of the virus in the cardamom tract of south India are discussed.


Assuntos
Regiões 3' não Traduzidas/classificação , Sequência de Aminoácidos , Proteínas do Capsídeo/classificação , Variação Genética , Índia , Dados de Sequência Molecular , Vírus do Mosaico/genética , Filogenia , Vírus de Plantas/genética , Alinhamento de Sequência , Análise de Sequência de RNA
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